The Z300 Handy-FluorCam is designed for chlorophyll fluorescence imaging in the field and laboratory. Typical experimental objects are small leaves, parts of leaves, small plants, algal or cyanobacterial colonies on stones, wood or soil, with areas of 3 x 3 cm or less. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols. The FluorCam software package includes a Wizard with the most frequently used experimental protocols. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences. The essential features of the instrument are similar to the Z100 Closed FluorCam System.
The Z300 HandyCam is easily portable with optional batteries  or Ultra light, in a convenient bag carried on the shoulder, and has several useful accessories. HandyCam generates images of fluorescence signal at any moment of the experiment and presents them using a false color scale. Full kinetic analysis is available. Handy-FluorCam is used to detect heterogeneity in fluorescence emission (F0, FM, FV, F0', FM', FV') or in parameters that are calculated from the fluorescence emission (e.g., NPQ, FV/FM, FV'/FM', Rfd, qN, qP). The information can be used to investigate heterogeneity within a single plant or leaf (e.g., infection, stating necrotic changes, senescence, abiotic stress) or to see differences among many plants (e.g., algal / cyanobacterial colonies, small canopy).

Z300 HandyCam Accessories
Handy Leaf Clip
The Handy Leaf Clip assembly is built specifically for use with the portable Handy FluorCam chlorophyll fluorometer. The clip simplifies the process of collecting fluorescence images on vegetation, allowing for dark adaptation and minimizing sample movement. The unique design includes a gentle trigger lock/release gripping system for holding the target leaf in place without detachment or excessive disturbance.   

 

Ultra Light Tripod
The Tripod Stand is a very useful accessory for field experiments. Its robust and flexible construction offers needed stability during imaging operations. The stand is water resistant and its variable height allows imaging of samples at different levels within the vegetation. Height up to 1.5 m; weight 3.3 kg; includes a bag. 

 

Experiments and Measured Parameters
QA reoxidation
Quenching
Kautsky induction and OJIP
Fast fluorescence induction with 1us resolution
Light Curves
Measured parameters: Fo, Fm, Fv, Fo', Fm', Fv', QY(II), Abs PAR-value
More than 50 calculated parameters including: NPQ, Fv/Fm (Maximum efficiency of photosynthesis), Fv'/Fm', Rfd, qN, qP, PAR-absorptivity, photosynthetic electron transport rate (PS) and others

Applications
Screening for photosynthetic performance
Heterogeneity investigation within a single plant or leaf, e.g., for infection, necrotic changes, senescence, abiotic stress.
Investigation for differences among many organisms, e.g., algal or cyanobacterial colonies, small plant canopies.
Growth and yield.

Imaging
CCD format: 512 x 512, A/D: 12 bit, 50 images per second (standard)
CCD format: 640 x 480, A/D: 12 bit, 30 images per second (optional)
CCD format: 1392 x 1040, A/D: 12 bit, 15 images per second (optional)

NEW FluorCam 6.0 Software
Automated experimental protocols via a Windows Wizard
Multiple (automatically repeated) experiments
Automated labeling of individual plants, or samples, within the field of view
Kinetic analysis of data from all samples within the field of view
Numerous image manipulation tools
Barcode reader support
Export to Excel
Windows 2000, XP, Vista

Producing Area:Canada