This unique device, only available from Qubit Systems, greatly increases the success rate of micro-electrode production in the lab. The silanizer facilitates the simultaneous silanization of six single barrels or six multi-barrel liquid ion-selective electrodes. Effective hydrophobicity, as induced through silanization, is essential for ensuring the successful introduction and retention of liquid ion exchange resin within the tip of the ion-selective barrel. Most procedures for micro-electrode construction and silanization have an extremely high failure rate. The microelectrode silanizer achieves a typical success rate of over 90%.
A major difficulty in the construction of double (or multi-barrel) ion-selective electrodes has been to selectively silanize only one barrel while leaving the reference barrel hydrophilic, and thus free of ion exchange resin. The micro-electrode silanizer overcomes this difficulty and allows for simultaneous silanization of batches of ion selective electrodes, making construction faster, easier and much more reliable.
Construct glass electrodes as described in the Silanizer manual. Attach up to 6 electrodes to the silanizer.
Place the silanizer into an oven for one hour at 200oC.
In a fume hood, inject DMDCS into the hot silanizer. As DMDCS is vaporized, silane is forced into the exposed ion selective barrels.
Cool and store the electrodes in a desiccatorto ensure complete evaporation of solvent.
24 hours after silanization, the electrodes are ready to be filled with ion exchanger. Use a flexible 34 AWG Microfil tip (World Precision Instruments) on a 5.0 mL syringe to back-fill the electrolyte, followed by application of pressure to force solution to the tip. Front fill the liquid ion exchanger (LIX) by immersing the entire electrode tip in LIX.
Producing Area:Canada