The Concept

Qubit Systems's package for measuring dissolved O2 has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

For many O2 electrodes, changing the membrane is a tedious and awkward process. Not so with Qubit Systems's O2 electrode. The membrane is located under the cuvette, which screws onto the base plate, and requires only a few seconds to change. All materials for replacing the membrane (filter paper and membrane material) are included in the Dissolved Oxygen Package.

Using Logger Pro software, the O2 electrode may be calibrated in units of mg O2/L, % dissolved O2, ppm, or whatever units are appropriate for your study. The Dissolved Oxygen Package provides excellent accuracy and a resolution of 0.1% O2 (where air saturated water = 100%). Dissolved O2 values may be expressed in any desired units (mg/L; % dissolved O2; ppm etc) based on a simple calibration procedure. The water jacket allows calibrations and experiments to be carried out at the same temperature, or a simple temperature correction can be made in software for measurements made at different temperatures.

Optional extras for the dissolved O2 package include a thermistor that monitors the temperature of the aqueous sample within the cuvette, a variable light source for illuminating photosynthetic samples, a light sensor for measuring irradiance levels, and syringes for introducing solutions into the cuvette during an experiment.

Applications

The Dissolved O2 Package may be used for any application that involves measurement of O2 in aqueous solutions. It can be used in the laboratory for measurement of O2 concentrations from samples taken from rivers and lakes, or to measure O2 consumption (or production) by living organisms. The dissolved O2 package is a perfect complement to Qubit Systems' Respiration, Plant CO2 Analysis and Photosynthesis Packages. While these packages are designed to measure the exchange of respiratory and photosynthetic gases in the gas phase, the O2 electrode allows similar measurements to be made on aquatic organisms, and also on isolated organelles, such as mitochondria and chloroplasts.

Experiments Include:

Measurement of Aquatic Photosynthetic O2 Evolution
Effect of Inhibitors on Mitochondrial Electron Transport
Analysis of Light-dependent Chloroplast Electron Transport
Effects of Temperature on O2 Uptake by Aquatic Animals
Kinetics of O2-consuming Enzymes

Oxygen Cuvette Electrode Base
 
The OX1LP-30mL Package Contains:

G109 Dissolved Oxygen Cuvette Electrode (30 mL)
A231 Cuvette Electrode Amplifier
A255 Magnetic Stirrer
A135 Stir Bar (2) and O-Ring (2)
A131 KCl Filling Solution
A136 Membrane Material and Filter Paper
10 ft Excelon Tubing for the Water Jacket
C410 LabPro Interface
C901 Logger Pro Software
C404 Customized Setup Software
Instructor's and Student's Manuals
Oxygen Cuvette Electrode Base
 

Optional S172 Thermistor

OX1LP Packages with various cuvette sizes

OX1LP-1mL - Dissolved Oxygen Package with 1 mL cuvette
OX1LP-4mL - Dissolved Oxygen Package with 4 mL cuvette
OX1LP-6mL - Dissolved Oxygen Package with 6 mL cuvette
OX1LP-50mL - Dissolved Oxygen Package with 50 mL cuvette

Optional Components:

S172 Thermistor to measure cuvette sample temperature
Syringes for addition of metabolites to cuvette; A141 (10µL), A143 (50µL), A145 (100µL)
A111 Variable halogen light source
S141 Light sensor

Producing Area:Canada