The Z125 Closed GFP FluorCam can serve for a number of applications using green fluorescent proteins in molecular and cellular biology. The basic system of the Closed FluorCam (CCD camera, four fixed LED panels) is supplemented by a fully motorized and software-controlled filter wheel and appropriate filter sets for the detection and imaging of green fluorescent protein (GFP), red-shifted GFP, or blue-emitting protein variants. The device configuration also provides user-friendly and quick transition between the GFP and chlorophyl fluorescence detection and imaging.
Applications
Gene transfer and biosensoring
Screening for genotype resistance
Plant-microbe interactions research
Bacterial-protozoan interactions research
Agriculture and horticulture
Experiments and Measured Parameters
Quenching
Kautsky induction
QA reoxidation (needs optional accessories)
OJIP (needs optional accessories)
Fast fluorescence induction with 1µs resolution (needs optional accessories)
PAR absorptivity (needs optional accessories)
Key Features
Ultra-high sensitivity CCD camera. Full 512 x 512 pixel resolurion; A/D: 12 bit; 50 images per second
Four super-bright blue LED panels (488 nm)
Additional infrared light source (optional)
4 or 8 position filter wheel - motorized and software-controlled
Selectable shelf system for different plant sizes
Dark room for adaptation
Coming with a high-end computer and user-friendly software
Samples
Leaves, plants, fruits, vegetables
Cyanobacteria, green algae
Sample size up to 13 cm x 13 cm
GFP Expression in Corn Kernels (GFPCam Captured)
GFPCam Inside View: Camera + LED Panels
Filter sets for the most commonly used GFP proteins
Proteins of the GFP family demonstrate a great spectral diversity and they often have different excitation and emission spectra. Therefore it is important to use the most appropriate filter sets so as to obtain maximum sensitivity for GFP viewing.
Red-shifted variants (typified by EGFP)
Their single excitation peak is centered at about 488 nm and the emission peak is at 509 nm.Suggested filter set: 485/20 for excitation and 530/25 for emission.
Original (wild type) GFP variants (wtGPF)
Their single excitation peak is centered at about 395 nm and the emission peak is at 509 nm.Suggested filter set: 400/20 or 360/40 for excitation and 508/20 for emission.
Blue-emitting variants (typified by EBFP)
They have the excitation peak at about 380 nm and the emission peak near 460 nm.Suggested filter set: 360/40 for excitation and 460/40 for emission.
Producing Area:Canada