The leaf chamber accessories kit consists of an acetate grid, coloured filters and vacuum grease.
A small container of vacuum grease is supplied to assist in sealing the leaf chamber. To prevent gas leaks from the chamber, the neoprene gaskets should be coated with a very thin layer of the vacuum grease.
The translucent acetate grid is divided into squares each 0.25 cm2 in area. The grid is used to measure leaf area by a statistical method.
The red, green and blue coloured filters provide light enhanced in their regions of the spectrum, sufficient to demonstrate the action spectrum of photosynthesis. The absorbance characteristics of the filters are balanced so that at a given distance from the light source they allow approximately the same quantum flux to reach the leaf. Note that this is true only if Qubit's 50 Watt A111 Halogen Light Source is used.

LEAF CHAMBER ACCESSORIES KIT

The G104 Arabidopsis Chamber consists of 2 pieces of Plexiglas bolted together and separated by gas-tight neoprene gaskets. Spacers placed on the bolts prevent the neoprene from being over-compressed, and also ensure that the chamber has a fixed inner volume. The chamber encloses a 3 cm2 area of an attached or detached arabidopsis leaf, or the leaf of any other small plant.
Gas is supplied to the chamber via tubing that branches into 2 gas inlet ports; one positioned to the side of the upper chamber lid, and the other to the side of the lower chamber lid. Gas passes from these ports into a manifold that directs it through small holes across the upper and lower surface of the leaf. This creates a turbulent flow that minimizes leaf boundary layer resistance. Gas leaves the chamber via small holes in the side of the chamber opposite the gas entry point. The gas enters a manifold in the upper and lower lids of the chamber, and passes from these into tubing that carries it to the drying column and the IRGA.
The G104 leaf chamber may be fitted with Qubit's S171 Leaf Temperature Thermistor to measure temperature throughout an experiment. Note that the temperature is only monitored and that it is not controlled. Care should be taken concerning the radiant heat load on the leaf while it is in the chamber, particularly when using the halogen light.
Sample Chamber Specifications:
Length = 2.0 cm
Width = 1.5 cm
Height = 1.0 cm
Volume = 3.0 mL

ARABIDOPSIS LEAF CHAMBER

The G111 Closed System Leaf Chamber is supplied as part of the Photosynthesis Package. The leaf chamber consists of two pieces of Lexan bolted together and separated by gas-tight neoprene gaskets. Spacers placed on the bolts prevent the neoprene from being over-compressed, and also ensure that the chamber has a fixed inner volume. To prevent gas leakage into and out of the chamber, the neoprene gaskets should be coated with a very thin layer of the vacuum grease that is supplied with the package.
The gas port fittings, and the O2 sensor, incorporated into the upper lid of the leaf chamber are attached securely to the Plexiglas, and the chamber is designed to provide years of trouble-free service. However, it should be noted that excessively rough handling of the chamber could cause components to break off, or to create cracks in the Plexiglas. The chamber should therefore be handled with care at all times.

CLOSED SYSTEM LEAF CHAMBER

Qubit's G112 Leaf Chamber may be used for open flow measurements of photosynthetic carbon dioxide exchange and is supplied as part of the Plant CO2 Analysis Packages.
The leaf chamber consists of 2 pieces of Lexan bolted together and separated by gas-tight neoprene gaskets. Spacers placed on the bolts prevent the neoprene from being over-compressed, and also ensure that the chamber has a fixed inner volume.
Gas is supplied to the chamber via tubing that branches into 2 gas inlet ports; one positioned to the side of the upper chamber lid, and the other to the side of the lower chamber lid. Gas passes from these ports into a manifold that directs it through small holes across the upper and lower surface of the leaf. This creates a turbulent flow that minimizes leaf boundary layer resistance. Gas leaves the chamber via small holes in the side of the chamber opposite the gas entry point. The gas enters a manifold in the upper and lower lids of the chamber, and passes from these into tubing that carries it to the drying column and the IRGA.
The G112 Leaf Chamber may be fitted with Qubit's S171 Leaf Temperature Thermistor to measure temperature throughout an experiment. Note that the temperature is only monitored and that it is not controlled. Care should be taken concerning the radiant heat load on the leaf while it is in the chamber, particularly when using the halogen light.
Sample Chamber Specifications:
Length = 3.0 cm
Width = 3.0 cm
Height = 1.0 cm
Volume = 9.0 mL

FLOW-THROUGH LEAF CHAMBER

Producing Area:Canada