Protocol Update for FluorPen
An additional feature which allows analysis of fluorescence induction curve and related fluorescence parameters.
|
PARAMETER |
VALUE
|
|
Bckg
|
1
|
|
Fo
|
430
|
|
Fj
|
1181
|
|
Fi
|
1712
|
|
Fm
|
1763
|
|
Fv
|
1333
|
|
Vj
|
0.563
|
|
Vi
|
0.962
|
|
Fm/Fo
|
4.1
|
|
Fv/Fo
|
3.1
|
|
Fv/Fm
|
0.756
|
|
Mo
|
0.861
|
|
Area
|
416115
|
|
Fix Area
|
1751702
|
|
Sm
|
312.165
|
|
Ss
|
0.654
|
|
N
|
477.184
|
|
Phi_Po
|
0.756
|
|
Psi_o
|
0.437
|
|
Phi_Eo
|
0.33
|
|
Phi_Do
|
0.244
|
|
Phi_Pav
|
977.657
|
|
Pi_Abs
|
1.188
|
|
ABS/RC
|
2.022
|
|
TRo/RC
|
1.529
|
|
ETo/RC
|
0.667
|
|
DIo/RC
|
0.493
|
Explanation of OJIP Parameters in the FluorPen:
Bckg = background
Fo: = F50µs; fluorescence intensity at 50 µs
Fj: = fluorescence intensity at j-step (at 2 ms)
Fi: = fluorescence intensity at i-step (at 60 ms)
Fm: = maximal fluorescence intensity
Fv: = Fm - Fo (maximal variable fluorescence)
Vj = (Fj - Fo) / (Fm - Fo)
Fm / Fo = Fm / Fo
Fv / Fo = Fv / Fo
Fv / Fm = Fv / Fm
Mo = TRo / RC - ETo / RC
Area = area between fluorescence curve and Fm
Sm = area / Fm - Fo (multiple turn-over)
Ss = the smallest Sm turn-over (single turn-over)
N = Sm . Mo . (I / Vj) turn-over number QA
Phi_Po = (I - Fo) / Fm (or Fv / Fm)
Phi_o = I - Vj
Phi_Eo = (I - Fo / Fm) . Phi_o
Phi_Do = 1 - Phi_Po - (Fo / Fm)
Phi_Pav = Phi_Po - (Sm / tFM); tFM = time to reach Fm (in ms)
ABS / RC = Mo . (I / Vj) . (I / Phi_Po)
TRo / RC = Mo . (I / Vj)
ETo / RC = Mo . (I / Vj) . Phi_o)
DIo / RC = (ABS / RC) - (TRo / RC)
Producing Area:Canada