LEAF CHAMBER FLUORCAM

LEAF CHAMBER FLUORCAM

The unique Leaf Chamber FluorCam has all the imaging features of the other instruments in the FluorCam family, but is designed for attachment to a leaf chamber so that chlorophyll fluorescence imaging can be done at the same time as gas exchange measurements. Other chlorophyll fluorometers that may be attached to leaf chambers provide only average values for the leaf within the chamber, or may provide data for only a small section of that leaf. The Leaf Chamber FluorCam allows the researcher to image the entire sample, and investigate heterogeneity. A great advantage of the Leaf Chamber FluorCam is that the leaf chamber can be used to control the environment of the sample precisely with respect to temperature, relative humidity, and the partial pressures of various gases that influence photosynthesis and transpiration (CO2, O2, water vapour).
The Leaf Chamber FluorCam may be attached to standard chambers provided by manufacturers of leaf gas exchange systems (ADC, LiCor, PP Systems etc.) or can be adapted for use with other chambers. Fluorescence emission is induced by sets of super-bright orange (blue) LEDs that provide measuring flashes. The photochemistry is driven by orange (blue) continuous actinic light and/or strong pulses of actinic light. The FluorWin software automatically generates sequences of fluorescence images for easy visualization of photosynthetic differences between and within samples. Values are also presented numerically.

FluorCam measures
Maximum efficiency of photosynthesis (FV/Fm)
Operating efficiency of photosynthesis (Fq'/Fm', PSII)
Non-photochemical quenching
Induction transients etc.

FluorWin Software Provides
Full control of timing, duration, and intensity of the light sources as well as camera operation
Automated experimental protocols via the FluorWin Wizard
Numerous image manipulation tools
Performing intelligent image segmentation and displaying fluorescence kinetics of the selected image segments
Using automated labeling of samples or selected segments
Combining images taken in the different phases of transients by the use of arithmetic operations, such as, subtraction, addition, or division. FV, FV/FM, qP, qN, NPQ, Rfd, and other fluorescence parameters can be calculated
Graphic and/or numeric presentation of measured data

Producing Area:Canada