Hand- made micro respiration chambers consisting of two parts:

  a custom-sized glass chamber of variable volume (500 - 5000 µl), inner diameter is 11.2 mm
  a 3-port glass stopper made for intermittent flow respirometry using fiber-optic needle-type sensors (O2 or pH) or other micro sensors.

Measuring Principle

One port is for the micro sensor and is made as an open capillary tube (0.75 ×14 mm) so tiny that it effectively prevents diffusion of air if filled with water or oil. The other two ports are for flushing the chamber intermittently with a peristaltic pump. both of these are fitted with pieces of steel needle for tygon tubes.
Fragile samples are separated from the glass-coated magnetic stir bar, by placing a piece of stainless steel net (mesh size 250 µm) on top of a glass spacer O-ring.

Applications

  Oxygen consumption of individual eggs, embryos and larvae (invertebrates, fish)
  Net oxygen evolution and respiration rates of phytoplankton cultures of low density
  Other applications with very low oxygen fluxes

Features

  Volumes in the range of ½ - 5 mL are available to fit different respiration or photosynthetic rates
  Designed for intermittent flow respirometry for long-term experiments with high temporal resolution and sensitivity
  No materials acting as oxygen sources or sinks
  Chamber and stopper made in borosilicate glass to give a tight seal and prevent diffusion of oxygen between the chamber and surroundings
  Designed for use with oxygen micro sensors featuring no O2 consumption, low response time, no stir effect, no cross-sensitivity, no electrical interference, sterilizable (H2O2, EtOH) etc.

NB: every chamber is handmade from borosilicate glass and therefore, their exact volumes have to be determined individually!

Included (D602)

  One custom-sized glass chamber
  One 3-port glass stopper
  One glass spacer
  One glass coated magnetic stir bar
  Stainless steel net for separation of magnet and fragile samples, 10 cm2

Producing Area:Denmark