Qubit Systems produces the only complete experimental package Q-Box NF1LP for measurement of nitrogen fixation in H2 producing legume symbioses. The unique flow-through H2 sensor (Q-S121) measures the production rate of H2 from N2-fixing tissues, allowing in vivo measurement of nitrogenase activity in real time. Measurement of H2 evolution as a means of determining nitrogenase activity overcomes all the problems of the traditional acetylene reduction assay. The Q-Box NF1LP Nitrogen Fixation Package is perfectly safe, non-invasive and allows changes in nitrogenase activity to be observed as they occur.
The Q-Box NF1LP Nitrogen Fixation Package includes:
- Q-S121 Hydrogen Analyzer (0-100ppm) includes drying column
- Q-S102 O2 Analyzer 0-100%
- C301 Event Switch (Air/Ar)
- S132 Temperature Sensor
- Q-G267 Pump & Flow Monitor (1L/min)
- Q-G268 Pump & Flow Monitor (2L/min)
- G122 Large Gas Bags (2)
- G132 Plant Growth Pots (4) with lids, fittings and Qubitac
- G161 Nodule Cuvette
- B201 Soybean seeds with Bradyrhizobium japonicum inoculant
- C610 LabQuest mini (2) data interface system
- C901 Logger Pro software
- C404 Customized Setup Software
- Rugged water-proof case housing all analyzers and sensors
- Q-Box Accessory kit
- Manual
- A248 Battery pack(Optional)
The Nitrogen Fixation Package contains everything required to conduct numerous investigations into the physiology and biochemistry of N2 fixation in an open-flow gas exchange system. We even supply legume seeds and inoculum! You supply only the computer. Calibration of analyzers is simple, set-up time is minimal and experiments are easy to perform.
Applications:
- Examine the effects of temperature on nitrogenase activity
- Examine the oxygen regulation of N2 fixation
- Measure the nitrogenase electron allocation coefficient
- Examine the relationship between N2 fixation and photosynthate supply
- Determine the effect of N2 fixation inhibition by fertilizers
software – data collection page:
software – data calculation page: